This book is comprises 4 units consisting General Veterinary Biochemistry, Intermediary Metabolism, Clinical Biochemistry and Animal Biotechnology containing 69 chapters covering complete VCI syllabus. Special mention has made to introduce to the reader some recent advanced molecular techniques. The book contains predigested information on basic principles of biochemical and molecular techniques for better understanding, assimilation and reproducibility. The compilation in the book may serve as a unique source and one point solution for various diagnostic tests of veterinary importance. Starting from the simple to most complex and advanced methods of diagnosis, the book has explained and elaborated in detail the mechanisms and clinical importance of different disease conditions which will be extremely helpful for students, academicians, researchers and clinicians. In addition, our main target was to make the book clinically-oriented. We earnestly hope that the book will be of help to all level of veterinary professionals.